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KMID : 1098419970050010007
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Korean Journal of Medicinal Crop Science
1997 Volume.5 No. 1 p.7 ~ p.13
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Effect of Plant Growth Regulators on the Adventitious Root Formation from Bupleurum falcatum Callus
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Sung Lak-Sun
Cho Duck-Yee
Soh Woong-Young
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Abstract
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Calli induced from the leaf segment of Bupleurum falcatum were cultured on Mu-rashige and Skoog’’s(MS) medium supplemented with 2, 4-D, IBA, IAA and NAA of 0.1 mg/l , The induction of adventitious roots from callus was the best in MS medium supplemented with 0.1 mg/l 2, 4-D and the lateral root was the same. The pretreatment of 0.1 mg/l 2, 4-D for 120 hours was most effective for the formation and grwoth of adventitious roots. The number of adventitious roots per micro callus pre-treated with 0.1 mg/l 2, 4- D was 5. 3 which was the highest level. The callus subcultured for 4 weeks were best for the adventitious root formation. The callus subcultured for more than 4 weeks decreased the adventitious root formation and turned to brown in color.
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KEYWORD
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Bupleurum falcatum, Adventitious Root, Plant Growth Regulators
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